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A-Level Biology OCR A

Rate Enzyme Increasing Increases Substrate Explain Conc Affects

Increasing enzyme concentration increases reaction rate by providing more active sites, until substrate becomes the limiting factor.

Increasing enzyme concentration increases the reaction rate by providing more active sites for substrate binding, until the substrate becomes limiting.

Front Explain how increasing enzyme conc. affects the initial rate of an enzyme-controlled reaction.
Back Increasing enzyme concentration:
  • As enzyme concentration increases, more active sites are available and reaction rate increases
  • This leads to more frequent successful collisions between substrate and enzyme
  • This increases the rate of formation of ES complexes
  • Therefore, rate increases
  • Rate stops increasing when substrate starts to run out and enzymes collide more often with each other than with substrate
  • At this point enzyme is no longer limiting and increasing conc will not affect rate (Vmax) because there will be nothing for enzymes to bind to 

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