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A-Level Biology OCR A

Dna Electrophoresis Strands Describe Events Stage Profiling Separating

The flashcard asks to describe the events in the third stage of DNA profiling, which involves separating DNA fragments using electrophoresis, subsequently separating double strands into single strands using alkali, and transferring them onto a membrane via Southern blotting.

Vyzýva na opis udalostí v tretej fáze profilovania DNA, ktorá zahŕňa separáciu DNA fragmentov pomocou elektroforézy, následné oddelenie dvojitých vlákien na jednotlivé pomocou zásady a prenos na membránu pomocou Southern blotting.

Front Describe the events in the third stage of DNA profiling.
Back Separating DNA fragments:
  • Cut fragments are separated to form a clear pattern using electrophoresis 
  • Electrophoresis gel is then immersed in alkali to separate double strands into single strands which are then transferred onto membrane by Southern blotting

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