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Dna Probes Complementary Describe Events Fourth Stage Profiling

The fourth stage of DNA profiling, hybridization, involves adding radioactive or fluorescent DNA probes that bind to specific microsatellite regions within DNA fragments on a membrane. This hybridization process helps identify these variable regions within the DNA.

La quarta fase del DNA profiling, l'ibridazione, comporta l'aggiunta di sonde di DNA radioattive o fluorescenti che si legano a specifiche regioni di microsatelliti nei frammenti di DNA su una membrana. Questo processo di ibridazione aiuta a identificare queste regioni variabili all'interno del DNA.

Front Describe the events in the fourth stage of DNA profiling.
Back Hypbridisation:
  • Radioactive/fluorescent DNA probes (short DNA/RNA sequences complementary to known DNA sqeuence) added in excess to DNA fragments on membrane
  • Probes bind to complementary strands under particular pH and temp - hybridisation
  • They are used to identify the microsatellite regions that vary more than minisatellites

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