A-Level Biology OCR A
Dna Temperature Strands Bases Primer Polymerase Machine Describe
PCR amplifies DNA by repeating cycles of denaturation, primer annealing, and extension by DNA polymerase, exponentially multiplying the desired DNA sequence.
La PCR amplifica el ADN mediante la repetición de ciclos de desnaturalización, unión de cebadores y extensión por ADN polimerasa, multiplicando exponencialmente la secuencia de ADN deseada.
| Front |
Describe how DNA can be amplified using PCR. |
| Back |
PCR:- Excess of four nucleotide bases (deoxynucleoside triphosphates), primer DNA sequences and DNA polymerase mixed in a vial & placed in PCR machine
- Temperature within machine is controlled and changes rapidly at programmed intervals
- At 95*C, the hydrogen bonds in DNA denature & strands separate
- Temperature then decreased to 55-60*C where primers bind to ends of DNA strands
- Temperature increased to 72-75*C where DNA polymerase (taq polymerase) adds bases to the primer, building up complementary strands of DNA and producing double stranded DNA identical to original sequence
- Cycle repeats over and over again until giving billions of copies of DNA to carry out DNA profiling
|
Learn with these flashcards. Click next, previous, or up to navigate to more flashcards for this subject.
Next card:
Fragments gel dna move electrophoresis current smaller larger
Previous card:
Dna define primer short nucleic acid sequence starting
Up to card list:
A-Level Biology OCR A