Apedia

A-Level Biology OCR A

Fragments Gel Dna Move Electrophoresis Current Smaller Larger

Gel electrophoresis separates DNA fragments based on their length. Negatively charged DNA fragments move through a gel matrix towards a positive anode when an electric current is applied. Smaller fragments move faster and farther than larger fragments.

L'elettroforesi su gel separa i frammenti di DNA in base alla loro lunghezza. I frammenti di DNA, caricati negativamente, si muovono attraverso una matrice di gel verso un anodo positivo quando viene applicata una corrente elettrica. I frammenti più piccoli si muovono più velocemente e più lontano rispetto ai frammenti più grandi.

Front Describe how gel electrophoresis is used to separate DNA fragments of different lengths.
Back Gel electrophoresis:
  • DNA fragments put into gel strips that contain buffer solution that maintains constant pH
  • Electric current is passed through electrophoresis plate, DNA fragments in wells at cathode end move through gel towards positive anode (due to negatively charged phosphate groups in DNA)
  • Rate of movement depends on mass/length of DNA fragments
  • Gel has mesh-like structure that makes it easier for smaller fragments to move through than larger ones
  • Over time smaller fragments move further along than larger & when one reaches anode, current is switched off and the DNA profile pattern can be seen

Learn with these flashcards. Click next, previous, or up to navigate to more flashcards for this subject.

Next card: Genetic define engineering modification characteristics organism manipulating material

Previous card: Dna temperature strands bases primer polymerase machine describe

Up to card list: A-Level Biology OCR A