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A-Level Biology OCR A

Light Laser Scanning Confocal Microscope Specimen Specific Outline

A laser scanning confocal microscope scans a specimen with a focused light spot, detecting fluorescence only from the focal plane via a pinhole, allowing for detailed 3D imaging.

Un microscopio confocal láser escanea el espécimen con un punto de luz enfocado, detectando fluorescencia solo de la profundidad focal a través de un pinhole, lo que permite imágenes 3D detalladas.

Front Outline how a laser scanning confocal microscope works.
Back Laser scanning confocal microscope
  • Specimen is treated with fluorescent chemical to illuminate/highlight specific structures
  • A single spot of focused light of a specific wavelength is moved across a specimen
  • Fluorescence from the components labelled with dye is caused

  • Emitted light is filtered through a pinhole apretrure
  • Only light radieted from very close to the focal plane is detected

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