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A-Level Biology OCR A

Power Focus Objective Coarse Stage Slide Lens Adjust

To use a microscope, focus first at low power with the coarse knob, center the object, then switch to high power objective, using only the fine focus for adjustment.

Para usar un microscopio, se enfoca primero a baja potencia con el tornillo macrométrico, luego se centra el objeto y se cambia al lente de alta potencia, usando solo el tornillo micrométrico para el ajuste fino.

Front Explain how to use a light microscope to view a specimen at low and high powers.
Back Using light microscope at lower power
  • Use coarse focus knob to give a maximum distance between stage and LP objective
  • Place a slide on the stage, as centrally over hole as possible
  • Switch power on, adjust brightness
  • Use coarse focus to move objective to as close to the slide as possible without smashing it
  • Now its safe to look thru eyepiece lens
  • Adjust illumimnation if needed, use coarse focus to slowly increase distance as you look thru
  • Use fine focus once you an see something to sharpen image
  • Move stage and/or slide around if needed

Changing to high power lens
  • Make sure there is something to see at center of your FOV - higher power lens covers smaller area of slide
  • Adjust brightness if needed
  • Look from side and move stage away from objective lens using coarse focus
  • Swing higher power objective into place
  • Repeat as if using lower power
  • Work your way up through objective lenses from lowest to highest magn so you can see specimen

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