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A-Level Biology OCR A

Rate Substrate Increasing Increases Concentration Explain Affects Initial

Increasing substrate concentration initially increases reaction rate, but it plateaus at Vmax when all active sites are occupied.

Increasing substrate concentration initially increases the reaction rate as more enzyme active sites are occupied, but the rate plateaus at Vmax when all active sites are saturated.

Front Explain how increasing the substrate concentration affects the initial rate of an enzyme-controlled reaction.
Back Increasing substrate concentration:
  • As substrate concentration increases, rate of successful colisions between substrate and enzyme increases
  • This increases the rate of formation of ES complexes
  • Therefore, rate increases
  • Rate stops increasing when all of the active sites are occupied
  • At this point substrate is no longer limiting and increasing conc will not affect rate (Vmax)

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