Temperature Beetroot Water Pieces Colorimeter Effect Permeability Cell

To study temperature's effect on membrane permeability, beetroot is exposed to different temperatures, and the absorbance of released pigment is measured using a colorimeter.

To investigate the effect of temperature on membrane permeability using a colorimeter, one would use beetroot pieces, expose them to varying temperatures in a water bath, and measure the absorbance of the released pigment.

Front

Describe a method using a colorimeter to investgate the effect of temperature on permeability of cell membranes.

Back

Investigating effect of temperature on permeability of cell membranes:

Cut five small pieces of beetroot of equal size

Wash beetroot pieces then place in 100ml distilled water in a water bath

Increase temperature of water bath in 10*C intervals

Take samples of water containing beetroot five mins after each temperature was reached

Measure absorbance of each sample using a colorimeter with a blue filter

Repeat experiment three times, each time with fresh beetroot pieces and calculate mean absorbance for each temperature

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