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A-Level Biology OCR A

Test Ethanol Beetroot Pieces Tube Colorimeter Effect Permeability

Front Describe a method using a colorimeter to investgate the effect of ethanol conc on permeability of cell membranes.
Back Investigating effect of ethanol concentration on permeability of cell membranes:
  • Cut five small pieces of beetroot of equal size
  • Prepare 5 solutions of ethanol in test tubes with concentrations 0%, 0.13%, 0.25%, 0.5%, 1%
  • Place one piece of beetroot in each test tube at the same time and start a timer for 5min, shake each test tube 3 times 
  • Once timer is done, remove the beetroot pieces from test tubes, shake each test tube 3 times and take sample of solution from each
  • Measure absorbance of each sample using a colorimeter with a blue filter
  • Repeat experiment each time with fresh beetroot pieces and calculate mean absorbance for each ethanol concentration

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