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A-Level Biology OCR A

Test Ethanol Beetroot Pieces Tube Colorimeter Effect Permeability

The impact of ethanol concentration on membrane permeability can be investigated by immersing beetroot in varying ethanol solutions and measuring the absorbance of the solutions.

Investigating the effect of ethanol concentration on membrane permeability involves exposing beetroot pieces to different ethanol solutions and measuring solution absorbance with a colorimeter.

Front Describe a method using a colorimeter to investgate the effect of ethanol conc on permeability of cell membranes.
Back Investigating effect of ethanol concentration on permeability of cell membranes:
  • Cut five small pieces of beetroot of equal size
  • Prepare 5 solutions of ethanol in test tubes with concentrations 0%, 0.13%, 0.25%, 0.5%, 1%
  • Place one piece of beetroot in each test tube at the same time and start a timer for 5min, shake each test tube 3 times 
  • Once timer is done, remove the beetroot pieces from test tubes, shake each test tube 3 times and take sample of solution from each
  • Measure absorbance of each sample using a colorimeter with a blue filter
  • Repeat experiment each time with fresh beetroot pieces and calculate mean absorbance for each ethanol concentration

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