| Front | Identification of host cells that have taken up the gene, using gene markers |
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| Back | marker genes can be inserted into vectors at the same time as the gene to be cloned Host cells grown on agar plates, each cell divides and replicates its DNA, creating a colony of cloned cells. Transformed cells will produced colonies where all the cells contain the cloned gene and the marker gene The marker gene can code for antibiotic resistance- host cells are grown on agar plates containing the specific antibiotic, so only transformed cells that have the marker gene will survive and grow. Marker gene can code for fluorescence when agar is placed under UV light, only transformed cells will fluoresce |
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