Transformation is the process of introducing recombinant DNA into host cells, often achieved by treating cells with calcium chloride and then heat-shocking them to facilitate plasmid uptake.
Transformation involves introducing recombinant DNA into a host cell (e.g., bacteria) using methods like heat shock after treating cells with calcium chloride to increase permeability.
Front | Transformation: inserting the vector into a suitable host |
---|---|
Back | Vector with recombinant DNA used to transfer gene into cells Host bacterial cells placed in ice-cold calcium chloride solution to make cell more permeable and the plasmids are added and mixture is heat shocked, (heated to around 45 degrees for 1-2 minutes) which encourages the cells to take up the plasmids Host cells that take up the vectors containing the gene of interest are said to be transformed |
Learn with these flashcards. Click next, previous, or up to navigate to more flashcards for this subject.
Next card: Cells gene marker host cloned agar transformed grown
Previous card: Dna recombinant produced combining sources
Up to card list: AQA Biology Flashcards