Transformation is the process of introducing recombinant DNA into host cells, often achieved by treating cells with calcium chloride and then heat-shocking them to facilitate plasmid uptake.
Transformation involves introducing recombinant DNA into a host cell (e.g., bacteria) using methods like heat shock after treating cells with calcium chloride to increase permeability.
| Front | Transformation: inserting the vector into a suitable host |
|---|---|
| Back | Vector with recombinant DNA used to transfer gene into cells Host bacterial cells placed in ice-cold calcium chloride solution to make cell more permeable and the plasmids are added and mixture is heat shocked, (heated to around 45 degrees for 1-2 minutes) which encourages the cells to take up the plasmids Host cells that take up the vectors containing the gene of interest are said to be transformed |
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