Chloroplasts can be isolated using differential centrifugation, spinning homogenised tissue at increasing speeds to separate them from cell debris.
Chloroplasts can be isolated from homogenised plant tissue using differential centrifugation, spinning at increasing speeds to pellet chloroplasts after removing cell debris.
| Front | Describe how a sample consisting only of chloroplasts could be obtained from homogenised plant tissue. ( 3 marks) |
|---|---|
| Back | use of differential centrifugation/or description first/low-spin pellet discarded / spin at low speed to remove cell wall material/cell debris supernatant re-spun at higher speed / until pellet with chloroplasts is found method of identifying chloroplasts e.g. microscopy; |
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