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Chloroplasts Pellet Speed Describe Sample Consisting Obtained Homogenised

Chloroplasts can be isolated using differential centrifugation, spinning homogenised tissue at increasing speeds to separate them from cell debris.

Chloroplasts can be isolated from homogenised plant tissue using differential centrifugation, spinning at increasing speeds to pellet chloroplasts after removing cell debris.

Front Describe how a sample consisting only of chloroplasts could be obtained from homogenised plant tissue. ( 3 marks) 
Back use of differential centrifugation/or description

first/low-spin pellet discarded / spin at low speed to remove cell wall material/cell debris

supernatant re-spun at higher speed / until pellet with chloroplasts is found

method of identifying chloroplasts e.g. microscopy;

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