TEMs provide high resolution for detailed cell imaging but cannot view living cells, require a vacuum, necessitate thin sections, and preparation can introduce artifacts or eliminate color.
TEMs offer high resolution and magnification for viewing small cell structures, but cannot be used on living cells, require a vacuum, involve thin sections, and preparations may introduce artifacts or lose color.
| Front | Scientists use optical microscopes and transmission electron microscopes (TEMs) to investigate cell structure. Explain the advantages and the limitations of using a TEM to investigate cell structure. (5 marks) |
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| Back | Advantages: Small objects can be seen; TEM has high resolution Wavelength of electrons shorter Limitations: Cannot look at living cells; Must be in a vacuum; Must cut section / thin specimen; Preparation may create artefact Does not produce colour image |
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