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Microscopes Investigate Cell Structure Tem Scientists Optical Transmission

TEMs provide high resolution for detailed cell imaging but cannot view living cells, require a vacuum, necessitate thin sections, and preparation can introduce artifacts or eliminate color.

TEMs offer high resolution and magnification for viewing small cell structures, but cannot be used on living cells, require a vacuum, involve thin sections, and preparations may introduce artifacts or lose color.

Front Scientists use optical microscopes and transmission electron microscopes (TEMs) to investigate cell structure. Explain the advantages and the limitations of using a TEM to investigate cell structure. (5 marks)
Back Advantages: 
Small objects can be seen; 

TEM has high resolution

Wavelength of electrons shorter

Limitations:
Cannot look at living cells; 

Must be in a vacuum; 

Must cut section / thin specimen; 

Preparation may create artefact 

Does not produce colour image

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