Reverse transcriptase synthesizes complementary DNA (cDNA) from mRNA templates, requiring isolation of mRNA and mixing it with nucleotides and the enzyme to create the cDNA strand.
Reverse transcriptase uses mRNA as a template to synthesize complementary DNA (cDNA). This process involves isolating mRNA, mixing it with cDNA nucleotides and reverse transcriptase, which then creates a DNA strand from the mRNA template.
| Front | Using reverse transcriptase to produce the DNA fragment |
|---|---|
| Back | mRNA used as templates to make lots of DNA Enzyme reverse transcriptase produces DNA from RNA, the DNA produced is complementary DNA (cDNA) mRNA isolated from cells and mixed with free floating DNA nucleotides and reverse transcriptase and then the reverse transcriptase uses the mRNA as a template to synthesise new strand of cDNA |
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