Recombinant DNA technology involves five key stages: isolating the gene of interest, inserting it into a vector, transforming a host organism, identifying successful transformants using gene markers, and finally, culturing the modified host cells.
The five stages of recombinant DNA technology are: 1. Isolating DNA fragments with the desired gene. 2. Inserting the fragment into a vector. 3. Transformation (inserting vector into host). 4. Identifying host cells with the gene using markers. 5. Culturing/cloning the host cells.
| Front | What are the 5 stages to recombinant DNA technology |
|---|---|
| Back | Stage 1: Isolation of DNA fragments that have the gene for the desired protein Stage 2: Insertion of DNA fragment into vector Stage 3: Transformation: Inserting the vector into a suitable host such as a bacterial cell Stage 4: Identification of host cells that have taken up the gene using gene markers Stage 5: Growth/Cloning of host cells |
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