The root tip squash experiment involves cutting the tip of a growing root, incubating it in hydrochloric acid at 60°C for 5 minutes to loosen cells, rinsing, breaking the tip to expose chromosomes, staining for better visibility, and squashing under a coverslip to thin the tissue for light penetration.
O experimento de esmagamento de ponta de raiz envolve cortar a ponta de uma raiz em crescimento, incubá-la em ácido clorídrico a 60°C por 5 minutos para soltar as células, enxaguá-la, quebrar a ponta para expor os cromossomas, manchá-la para melhor visualização e esmagá-la sob uma lamela para torná-la mais fina e permitir a passagem de luz.
| Front | What is the experiment for root tip squash |
|---|---|
| Back | 1. Cut 1cm from tip of growing root 2. have boiling tube containing 1 Mole hydrochloric acid and place in water bath at 60 3. transfer root tip into boiling tube and incubate for 5 minutes 4. rinse root with cold water and dry 5. use a mounted needle to break tip open 7. add stain to see chromosome easier 8. place coverslip and squash tissue making the tissue thinner so more light |
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