Using mRNA and reverse transcriptase is advantageous because the gene in the chromosome may contain introns, and prokaryotes cannot splice pre-mRNA, preventing them from using the gene to code for desired protein amino acid sequences.
Usar ARNm y transcriptasa inversa es ventajoso porque el gen en el cromosoma puede contener intrones, y los procariotas no pueden empalmar el ARNm pre, lo que les impide usar el gen para codificar secuencias de aminoácidos de proteínas deseadas.
| Front | What is the advantage in using mRNA and reverse transcriptase instead of a restriction endonuclease? |
|---|---|
| Back | - DNA in chromosome may contain introns - prokaryotes cannot splice pre-mRNA - so cannot use gene to code for amino acid sequence of desired protein |
Tags: exam, gene_technology
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