PCR amplifies DNA by repeatedly heating to separate strands, cooling for primer annealing and nucleotide binding, and raising temperature for DNA polymerase to synthesize new strands.
- DNA heated to 95°C - to break H bonds and separate strands - DNA cooled to 60°C - to allow primers to anneal to ends of DNA fragments - free DNA nucleotides bind to complementary DNA base - adenine with thymine and cytosine with guanine - temperature increased to 70°C - DNA polymerase forms phosphodiester bonds between free nucleotides - in condensation reactions - cycle is repeated to amplify amount of DNA
| Front | What happens in PCR? |
|---|---|
| Back | - DNA heated to 95°C - to break H bonds and separate strands - DNA cooled to 60°C - to allow primers to anneal to ends of DNA fragments - free DNA nucleotides bind to complementary DNA base - adenine with thymine and cytosine with guanine - temperature increased to 70°C - DNA polymerase forms phosphodiester bonds between free nucleotides - in condensation reactions - cycle is repeated to amplify amount of DNA |
Tags: gene_technology
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