| Front | How do you do transfer a gene into a vector? |
|---|---|
| Back | - cut desired gene from chromsome - using specific restriction endonuclease with complementary recognition sequence - produces sticky ends - cut plasmid using same restriction endonuclease - sticky ends attracted to each other by complementary base pairing - DNA ligase forms phosphodiester bonds between sticky ends of gene and plasmid - forming sugar-phosphate backbone - recombinant plasmid formed |
Tags: gene_technology
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