Question

Describe Transmission Electron Microscopy. (9)

Accepted Answer

- requires vacuum so live specimens cannot be observed

- complex staining process

- requires very thin specimen

- electron beam passed through specimen

- focuses using electromagnets

- parts that absorb electrons appear dark, parts that electron pass through appear bright

- resolution of 0.1 nm due to short wavelength of electron beam

- does not show colour

- 2-D image produced

Front	Describe Transmission Electron Microscopy. (9)
Back	- requires vacuum so live specimens cannot be observed - complex staining process - requires very thin specimen - electron beam passed through specimen - focuses using electromagnets - parts that absorb electrons appear dark, parts that electron pass through appear bright - resolution of 0.1 nm due to short wavelength of electron beam - does not show colour - 2-D image produced

Electron Requires Specimen Beam Parts Describe Transmission Microscopy