Transformed bacterial cells can be identified using marker genes (e.g., antibiotic resistance, fluorescence) which are either incorporated into or disrupted by the recombinant DNA.
- use antibiotic resistance/ fluorescent/ enzyme marker genes - recombinant DNA fragment may contain marker gene - recombinant DNA fragment may disrupt marker gene - so only transformed/untransformed cells will fluoresce/not fluoresce
| Front | How do you identify bacterial cells that have been transformed? |
|---|---|
| Back | - use antibiotic resistance/ fluorescent/ enzyme marker genes - recombinant DNA fragment may contain marker gene - recombinant DNA fragment may disrupt marker gene - so only transformed/untransformed cells will fluoresce/not fluoresce |
Tags: gene_technology
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