Bacterial cells are transformed by mixing them with recombinant plasmids in the presence of calcium ions and then applying a heat shock, which makes the cell membranes permeable, allowing plasmid uptake.
- place bacterial cells and recombinant plasmids in solution containing Ca2+ - use heatshock - bacterial cell mebrane becomes highly permeable - bacterial cells take up recombinant plasmid and are transformed
| Front | How do you cause transformation of bacterial cells? |
|---|---|
| Back | - place bacterial cells and recombinant plasmids in solution containing Ca2+ - use heatshock - bacterial cell mebrane becomes highly permeable - bacterial cells take up recombinant plasmid and are transformed |
Tags: gene_technology
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