Genetic engineering involves cutting DNA with restriction enzymes, opening a vector (like a plasmid) with the same enzyme, inserting the gene using ligase, and introducing the recombinant DNA into host cells.
Descreva as principais etapas da engenharia genética: 1. Recortar DNA com enzima de restrição. 2. Usar a mesma enzima para abrir o DNA do vetor. 3. Isso deixa pontas pegajosas com bases complementares onde foram cortadas. 4. Inserir o gene desejado no plasmídeo usando a enzima ligase. 5. DNA recombinante (novo vetor) é inserido em outras células, ex. bactérias. 6. As células se multiplicam rapidamente.
| Front | Describe the main stages of genetic engineering |
|---|---|
| Back | 1. Cut out DNA with restriction enzyme 2. Use same enzyme to cut open vector DNA 3. This leaves sticky ends with complementary bases where they were cut 4. Insert desired gene into plasmid using ligase enzyme 5. Recombinant DNA (new vector) is inserted into other cells e.g bacteria 6. Cells multiply rapidly |
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