Using the same restriction enzyme ensures complementary 'sticky ends' on both the DNA fragment and the vector, facilitating base pairing for insertion.
Por que a mesma enzima de restrição é usada para cortar o DNA e o DNA do vetor? Para produzir as mesmas pontas pegajosas complementares que se unem por pareamento de bases.
| Front | Why is the same restriction enzyme used to cut the DNA out and the vector DNA? |
|---|---|
| Back | To produce the same complementary sticky ends that join by base pairing |
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