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Solution Boiling Ph Put  Of Tube Drop Iodine

Front REQUIRED PRACTICAL : Investigating the effect of pH on enzyme activity
Back 1. Put a drop of iodine solution in a spotting tile. 
2. Using a bunsen burner, heat up a beaker of water to 35oC (thermometer)
3. Using a syringe add 3cm3 of amylase solution and 1cm3 of buffer solution with pH5 to a boiling tube. Put this boiling tube in the beaker of water and wait 5 minutes.
4. After 5 minutes, add 3cm3 of starch solution to the boiling tube (using a different syringe)
5. Immediately mix the contents and start a stopwatch.
6. Every 10 seconds, put a drop from the boiling tube into a spotting tile.
7. When the iodine solution remains browny-orange, there is no more starch present.
8. Repeat with different pH of buffer solution to see how pH affects rate of reaction.
9. Remember to control variables e.g concentration and volume of amylase solution.

Tags: biology_paper_1, biology_paper_2

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