| Front | Types of gel electrophoresis: Agarose (DNA separation) |
|---|---|
| Back | DNA cut into fragments using restriction endonuclease – different samples having different fragment lengths Fragments separate because DNA is negatively charged due to the presence of a phosphate group (PO43–) on each nucleotide DNA samples are placed into an agarose gel and fragment size calculated by comparing against known industry standards Specific sequences can be identified by incorporating a complementary radiolabelled hybridisation probe, transferring the separated sequences to a membrane and then visualising via autoradiography (Southern blotting) |
Tags: 3_5_genetic_modification, gel_electrophoresis
Learn with these flashcards. Click next, previous, or up to navigate to more flashcards for this subject.
Next card: Proteins gel polyacrylamide negative types electrophoresis separation folded
Previous card: Gel samples move stages electrophoresis block electric current
Up to card list: IB Biology HL