| Front | Types of gel electrophoresis: polyacrylamide (protein separation) |
|---|---|
| Back | Proteins may be folded into a variety of shapes (affecting size) and have positive and negative regions (no clear charge) Proteins treated with an anionic detergent to linearise and impart a uniform negative charge Protein samples are placed into a polyacrylamide gel and sizes compared against known industry standards Separated proteins are transferred to a membrane and then target proteins are identified by staining with specific monoclonal antibodies (Western blotting) |
Tags: 3_5_genetic_modification, gel_electrophoresis
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