Polyacrylamide gel electrophoresis separates proteins. Proteins are treated with a detergent to linearize and give them a uniform negative charge, as their natural structure and charge vary. Sizes are compared to standards, and specific proteins are identified using Western blotting with antibodies.
Polyacrylamide gel electrophoresis separates proteins. Proteins are treated with a detergent to linearize and give them a uniform negative charge, as their natural structure and charge vary. Sizes are compared to standards, and specific proteins are identified using Western blotting with antibodies.
| Front | Types of gel electrophoresis: polyacrylamide (protein separation) |
|---|---|
| Back | Proteins may be folded into a variety of shapes (affecting size) and have positive and negative regions (no clear charge) Proteins treated with an anionic detergent to linearise and impart a uniform negative charge Protein samples are placed into a polyacrylamide gel and sizes compared against known industry standards Separated proteins are transferred to a membrane and then target proteins are identified by staining with specific monoclonal antibodies (Western blotting) |
Tags: 3_5_genetic_modification, gel_electrophoresis
Learn with these flashcards. Click next, previous, or up to navigate to more flashcards for this subject.
Next card: Dna profiling is technique individuals identified compared respective
Previous card: Dna gel agarose fragments samples sequences types electrophoresis
Up to card list: IB Biology HL