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Gene Vector Pcr).  Digestion Of Restriction Endonuclease Ligation Dna

The process of gene transfer involves isolating the gene and vector (often using PCR), digesting both with restriction endonucleases, ligating the gene into the vector using DNA ligase, and finally selecting and expressing the transgenic construct.

The process of gene transfer involves isolating the gene and vector (often using PCR), digesting both with restriction endonucleases, ligating the gene into the vector using DNA ligase, and finally selecting and expressing the transgenic construct.

Original
of gene and vector (by PCR). 
Digestion of gene and vector (by restriction endonuclease)
Ligation of gene and vector (by DNA ligase)
Selection and expression of transgenic construct
Title What is the process of gene transfer? Isolation... 
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of gene and vector (by PCR). 
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of gene and vector (by PCR). 
Digestion of gene and vector (by restriction endonuclease)
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Digestion of gene and vector (by restriction endonuclease)
Ligation of gene and vector (by DNA ligase)
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Ligation of gene and vector (by DNA ligase)
Selection and expression of transgenic construct
Full
of gene and vector (by PCR). 
Digestion of gene and vector (by restriction endonuclease)
Ligation of gene and vector (by DNA ligase)
Selection and expression of transgenic construct

Tags: 3_5_genetic_modification, insulin

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