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IB Biology HL

Gene Sequences Dna Vectors  Modified Centrifugation Heavier Components

Original
1. centrifugation – heavier components like nuclei are separated
2. PCR - amplification of gene of interest. Gene sequences can also be generated from 
mRNA using reverse transcriptase – these DNA sequences (cDNA) lack introns. A vector is 
a DNA molecule that is used as a vehicle to carry the gene of interest into a foreign cell. Bacterial plasmids 
are commonly used as vectors because they are capable of autonomous self-replication and expression. These plasmids may be
 modified for further functionality (e.g. selection markers, reporter genes, inducible expression promoters). Other types of vectors include
 modified viruses and artificial chromosomes
Title What is the process of isolating gene and vector in gene transfer? 
Settings 1,1,0 | n,n,n,n
Text1
1. centrifugation – heavier components like nuclei are separated
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Text2
1. centrifugation – heavier components like nuclei are separated
2. PCR - amplification of gene of interest. Gene sequences can also be generated from 
...
...
...
...
...
Text3
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2. PCR - amplification of gene of interest. Gene sequences can also be generated from 
mRNA using reverse transcriptase – these DNA sequences (cDNA) lack introns. A vector is 
...
...
...
...
Text4
...
...
mRNA using reverse transcriptase – these DNA sequences (cDNA) lack introns. A vector is 
a DNA molecule that is used as a vehicle to carry the gene of interest into a foreign cell. Bacterial plasmids 
...
...
...
Text5
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...
...
a DNA molecule that is used as a vehicle to carry the gene of interest into a foreign cell. Bacterial plasmids 
are commonly used as vectors because they are capable of autonomous self-replication and expression. These plasmids may be
...
...
Text6
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...
...
...
are commonly used as vectors because they are capable of autonomous self-replication and expression. These plasmids may be
 modified for further functionality (e.g. selection markers, reporter genes, inducible expression promoters). Other types of vectors include
...
Text7
...
...
...
...
...
 modified for further functionality (e.g. selection markers, reporter genes, inducible expression promoters). Other types of vectors include
 modified viruses and artificial chromosomes
Full
1. centrifugation – heavier components like nuclei are separated
2. PCR - amplification of gene of interest. Gene sequences can also be generated from 
mRNA using reverse transcriptase – these DNA sequences (cDNA) lack introns. A vector is 
a DNA molecule that is used as a vehicle to carry the gene of interest into a foreign cell. Bacterial plasmids 
are commonly used as vectors because they are capable of autonomous self-replication and expression. These plasmids may be
 modified for further functionality (e.g. selection markers, reporter genes, inducible expression promoters). Other types of vectors include
 modified viruses and artificial chromosomes

Tags: 3_5_genetic_modification, insulin

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