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IB Biology HL

Vector Gene Inserted Plasmid Cut With  Restriction Endonucleases. This

Ligation involves inserting the gene into a similarly cut plasmid vector, allowing complementary sticky ends to base pair. The enzyme DNA ligase then splices the gene and vector together by forming covalent phosphodiester bonds in their sugar-phosphate backbones, creating a recombinant construct.

Ligation involves inserting the gene into a similarly cut plasmid vector, allowing complementary sticky ends to base pair. The enzyme DNA ligase then splices the gene and vector together by forming covalent phosphodiester bonds in their sugar-phosphate backbones, creating a recombinant construct.

Original
inserted into a plasmid vector that has been cut with 
the same restriction endonucleases. This occurs because 
the sticky ends of the gene and vector overlap via complementary base pairing. The gene and vector are then 
spliced together by the enzyme DNA ligase to form 
a recombinant construct. DNA ligase joins the 
vector and gene by 
fusing their sugar-phosphate backbones together with a 
covalent phosphodiester bond.
Title What is the process of ligation of vector and insert in gene transfer? The gene is ...
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inserted into a plasmid vector that has been cut with 
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Text2
inserted into a plasmid vector that has been cut with 
the same restriction endonucleases. This occurs because 
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Text3
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the same restriction endonucleases. This occurs because 
the sticky ends of the gene and vector overlap via complementary base pairing. The gene and vector are then 
...
...
...
...
...
Text4
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the sticky ends of the gene and vector overlap via complementary base pairing. The gene and vector are then 
spliced together by the enzyme DNA ligase to form 
...
...
...
...
Text5
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spliced together by the enzyme DNA ligase to form 
a recombinant construct. DNA ligase joins the 
...
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...
Text6
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a recombinant construct. DNA ligase joins the 
vector and gene by 
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...
Text7
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vector and gene by 
fusing their sugar-phosphate backbones together with a 
...
Text8
...
...
...
...
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fusing their sugar-phosphate backbones together with a 
covalent phosphodiester bond.
Full
inserted into a plasmid vector that has been cut with 
the same restriction endonucleases. This occurs because 
the sticky ends of the gene and vector overlap via complementary base pairing. The gene and vector are then 
spliced together by the enzyme DNA ligase to form 
a recombinant construct. DNA ligase joins the 
vector and gene by 
fusing their sugar-phosphate backbones together with a 
covalent phosphodiester bond.

Tags: 3_5_genetic_modification, insulin

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