Question

cut with restriction enzymes at specific recognition sites. Restriction enzymes cleave the sugar-phosphate backbone to generate sticky ends (complementary overhangs). Scientists will often cleave the vector and gene with two different ‘sticky end’ restriction endonucleases (double digestion) to ensure the gene is inserted in the correct orientation and to prevent the vector from re-annealing without the desired insert. 1,1,0 | n,n,n,n cut with restriction enzymes at specific recognition sites. Restriction enzymes cleave the ............... ......sugar-phosphate backbone to generate sticky ends (complementary overhangs). Scientists will often cleave the vector and gene with ......... ............two different ‘sticky end’ restriction endonucleases (double digestion) to ensure the gene is inserted in the correct orientation and to prevent ... cut with restriction enzymes at specific recognition sites. Restriction enzymes cleave the sugar-phosphate backbone to generate sticky ends (complementary overhangs). Scientists will often cleave the vector and gene with two different ‘sticky end’ restriction endonucleases (double digestion) to ensure the gene is inserted in the correct orientation and to prevent the vector from re-annealing without the desired insert.

Accepted Answer

What is the process of digestion with restriction enzymes in gene transfer? The gene and vector are  cut with restriction enzymes at .................. ...specific recognition sites. Restriction enzymes cleave the sugar-phosphate backbone to ............ .........generate sticky ends (complementary overhangs). Scientists will often cleave the vector and gene with two different ‘sticky end’ restriction endonucleases (double digestion) to ensure ...... ...............the gene is inserted in the correct orientation and to prevent the vector from re-annealing without the desired insert.

Original	cut with restriction enzymes at specific recognition sites. Restriction enzymes cleave the sugar-phosphate backbone to generate sticky ends (complementary overhangs). Scientists will often cleave the vector and gene with two different ‘sticky end’ restriction endonucleases (double digestion) to ensure the gene is inserted in the correct orientation and to prevent the vector from re-annealing without the desired insert.
Title	What is the process of digestion with restriction enzymes in gene transfer? The gene and vector are
Settings	1,1,0 \| n,n,n,n
Text1	cut with restriction enzymes at ... ... ... ... ... ...
Text2	cut with restriction enzymes at specific recognition sites. Restriction enzymes cleave the ... ... ... ... ...
Text3	... specific recognition sites. Restriction enzymes cleave the sugar-phosphate backbone to ... ... ... ...
Text4	... ... sugar-phosphate backbone to generate sticky ends (complementary overhangs). Scientists will often cleave the vector and gene with ... ... ...
Text5	... ... ... generate sticky ends (complementary overhangs). Scientists will often cleave the vector and gene with two different ‘sticky end’ restriction endonucleases (double digestion) to ensure ... ...
Text6	... ... ... ... two different ‘sticky end’ restriction endonucleases (double digestion) to ensure the gene is inserted in the correct orientation and to prevent ...
Text7	... ... ... ... ... the gene is inserted in the correct orientation and to prevent the vector from re-annealing without the desired insert.
Full	cut with restriction enzymes at specific recognition sites. Restriction enzymes cleave the sugar-phosphate backbone to generate sticky ends (complementary overhangs). Scientists will often cleave the vector and gene with two different ‘sticky end’ restriction endonucleases (double digestion) to ensure the gene is inserted in the correct orientation and to prevent the vector from re-annealing without the desired insert.

Gene Restriction Vector Enzymes Cut At Specific Recognition