Text | Hybridization is the joining of complementary base pair sequences, which can be DNA-DNA recognition or RNA-DNA recognition; next two things require it Polymerase Chain Reaction or PCR can produce millions of copies of DNA without amplifying the DNA in bacteria. It requires primers that are complementary to the DNA that flanks the region of interest, nucleotides, and DNA polymerase. Southern Blots are used to detect the presence and quantity of various DNA strands in a sample by cutting DNA with restriction enzymes and then separation through gel electrophoresis. In southern blots, probes are labeled with radioisotopes or indicator proteins, which can be used to indicate the presence of the desired sequence |
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Tags: biochemistry_6
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