Southern Blotting involves cutting DNA with restriction enzymes, separating it by gel electrophoresis, transferring it to a membrane, and probing with a labeled single-stranded DNA sequence that binds to complementary sequences, visualized by radioactivity.
Southern Blotting involves cutting DNA with restriction enzymes, separating it by gel electrophoresis, transferring it to a membrane, and probing with a labeled single-stranded DNA sequence that binds to complementary sequences, visualized by radioactivity.
| Text | Southern Blots 1) DNA cut by restriction enzymes 2) DNA separated by gel electrophoresis 3) DNA fragments are transferred to a membrane 4) membrane is probed with many copies of a single-stranded DNA sequence 5) The probe will bind to its complementary sequence and form double-stranded DNA 6) Radioisotope in probe will then identify the DNA |
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Tags: biochemistry_6
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