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Dna Membrane Sequence Probe Southern Blots Cut Restriction

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Southern Blots
1) DNA cut by restriction enzymes
2) DNA separated by gel electrophoresis
3) DNA fragments are transferred to a membrane
4) membrane is probed with many copies of a single-stranded DNA sequence
5) The probe will bind to its complementary sequence and form double-stranded DNA
6) Radioisotope in probe will then identify the DNA

Tags: biochemistry_6

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