Automated DNA sequencing utilizes fluorescent ddNTPs, combines reactions and electrophoresis in one lane, and employs a laser detector to record the sequence.
Automated DNA sequencing uses fluorescently labeled ddNTPs and performs all reactions and electrophoresis in a single lane, with a laser and detector recording the sequence.
| Front | Automated DNA sequencing mechanism |
|---|---|
| Back | Fluorescently labelled ddNTPs are used. All 4 reactions and the electrophoresis can be performed in the same lane. UV Laser beam and Photoelectric Detector moves from bottom to top and records the sequence (computer also produces peaks). |
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