PCR steps with temp and time

1) Denaturation:94°C for 1 minute2) Primer annealing:54°C for 2 minutes3) Polymerization:72°C for 1 minute

Minute Pcr Steps Temp Time Denaturation 94°C Primer

PCR involves denaturation at 94°C for 1 minute, primer annealing at 54°C for 2 minutes, and polymerization at 72°C for 1 minute.

PCR steps include Denaturation (94°C/1 min), Primer annealing (54°C/2 min), and Polymerization (72°C/1 min).

Front	PCR steps with temp and time
Back	1) Denaturation: 94°C for 1 minute 2) Primer annealing: 54°C for 2 minutes 3) Polymerization: 72°C for 1 minute

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