Thin Layer Chromatography (TLC) separates amino acids based on their affinity for the stationary phase and solubility in the mobile phase, with the retention factor (Rf) calculated by the distance traveled by the solute and solvent.
A Cromatografia em Camada Fina (TLC) separa aminoácidos com base em sua afinidade com a fase estacionária e solubilidade na fase móvel, com o fator de retenção (Rf) calculado pela distância percorrida pelo soluto e pelo solvente.
| Front | TLC 2.1.2(s) |
|---|---|
| Back | Mobile phase picks up AA and moves through stationary phase so AA separated AA added to silicon gel (stationary phase) and submerged in organic solvent (mobile phase) and thus AA separated Rate at which diff AA move depends on H2 bonds with silica (SP) and solubility in organic solvent (MP) Rf=distance moved by solute/distance moved by solvent |
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