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Aa Solvent Phase Separated  Organic Moved Tlc 2.1.2(s

Front TLC 2.1.2(s)
Back Mobile phase picks up AA and moves through stationary phase so AA separated 

AA added to silicon gel (stationary phase) and submerged in organic solvent (mobile phase) and thus AA separated 

Rate at which diff AA move depends on H2 bonds with silica (SP) and solubility in organic solvent (MP)

Rf=distance moved by solute/distance moved by solvent

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