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Divisions Micrometer Eyepiece Stage Graticule U Mag Factor 

Front Eyepiece graticules 2.1.1(b)
Back Calibrate EG for all objective lens by placing stage micrometer on stage and EG on eyepiece lens, align and see how many divisions on graticule form divisions on micrometer

Now use 100 divisions on micrometer = 1mm to find length in 1 division, and thus in micrometer divisions u are looking for, then use this and div by gractucule divisions u are looking for for mag factor 

Then replace micrometer with sample, and for actual size , do graticule divisions * mag factor 

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