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Mix N 14 N 15 Strand Free Nucleotides Centrifuged Cycle

This flashcard explains an experiment using N-14/N-15 isotopes and centrifugation to demonstrate semi-conservative DNA replication, showing how bands shift over generations.

This flashcard describes a density gradient centrifugation experiment using N-14 and N-15 isotopes to demonstrate semi-conservative DNA replication.

Front N-14/ N-15
Back old strand has N-14 and free nucleotides N-15, centrifuged, first cycle results in mix of 14 and 15

second gen mix of 14 and 15 and a band for just 15

shows semi-conservative rep

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