This flashcard explains an experiment using N-14/N-15 isotopes and centrifugation to demonstrate semi-conservative DNA replication, showing how bands shift over generations.
This flashcard describes a density gradient centrifugation experiment using N-14 and N-15 isotopes to demonstrate semi-conservative DNA replication.
Front | N-14/ N-15 |
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Back | old strand has N-14 and free nucleotides N-15, centrifuged, first cycle results in mix of 14 and 15 second gen mix of 14 and 15 and a band for just 15 shows semi-conservative rep |
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