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OCR A Biology

Test Antibodies Coloured Pregnant Line Urine Monoclonal Window

Tests for pregnancy by detecting hCG in urine. The color intensity in test windows indicates the presence of pregnant hormones, determined by antibody binding.

Prüft auf eine Schwangerschaft anhand der Urinkonzentration von hCG, wobei die Farbintensität durch Antikörperbindungen bestimmt wird, die in einem Testfenster angezeigt werden.

Front pregnancy test
Back
  • wick soaked in first urine passed in morning (highest levels of hCG)
  • test contains mobile monoclonal antibodies (with small coloured beads attached) which only binds to hCG, if pregnant, hCG binds and forms hCG/antibody complex (complete with coloured beads)
  • urine carries on along test structure until it reaches a window where immobalised monoclonal antibodies arranged in line or pattern that only bind to hCG/antibody complex, so if pregnant, coloured line/pattern appears in first window
  • urine continues to second window where theres a line of immobalised monoclonal antibodies that bind only to mobile antibodies (whether bound to hCG or not) so coloured line forms to indicate test working, so if woman pregnant, 2 lines, and if not pregnant, 1 line

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